DC Field | Value | Language |
dc.contributor.author | Mutsenko, V. | - |
dc.contributor.author | Ariana Barlic | - |
dc.contributor.author | Pezic, T. | - |
dc.contributor.author | Janja Dermol-Cerne | - |
dc.contributor.author | Katkov, I. I. | - |
dc.date.accessioned | 2021-06-28T07:44:37Z | - |
dc.date.available | 2021-06-28T07:44:37Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Me₂SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars / V. Mutsenko [et al.] // Cryobiology. - 2019. - Vol.91.-P. 104-114. - Doi: https://doi.org/10.1016/j.cryobiol.2019.10.002. | ru |
dc.identifier.uri | http://dspace.bsu.edu.ru/handle/123456789/42476 | - |
dc.description.abstract | Cryopreservation is the universal technology used to enable long-term storage and continuous availability of cell stocks and tissues for regenerative medicine demands. The main components of standard freezing media are dimethyl sulfoxide (hereinafter Me₂SO) and fetal bovine serum (FBS). However, for manufacturing of cells and tissue-engineered products in accordance with the principles of Good Manufacturing Practice (GMP), current considerations in regenerative medicine suggest development of Me₂SO- and serum-free biopreservation strategies due to safety concerns over Me2SO-induced side effects and immunogenicity of animal serum. In this work, the effect of electroporation-assisted pre-freeze delivery of sucrose, trehalose and raffinose into human umbilical cord mesenchymal stem cells (hUCMSCs) on their post-thaw survival was investigated | ru |
dc.language.iso | en | ru |
dc.subject | biology | ru |
dc.subject | developmental biology | ru |
dc.subject | Me2SO- and serum-free cryopreservation | ru |
dc.subject | electroporation | ru |
dc.subject | permeabilization | ru |
dc.subject | sugar | ru |
dc.subject | human umbilical cord | ru |
dc.subject | mesenchymal stem cells | ru |
dc.title | Me₂SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars | ru |
dc.type | Article | ru |
Appears in Collections: | Статьи из периодических изданий и сборников (на иностранных языках) = Articles from periodicals and collections (in foreign languages)
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