Optimization of methods of quality control drug steroid structure for example glucocorticosteroid

Abstract

This paper presents an original method for the analysis of corticosteroids with the use of reversed-phase high performance liquid chromatography. A mixture of 1% aqueous formic acid and ethyl alcohol at a ratio of 10 to 90 was taken as an eluting system for corticosteroids. This ratio of solvents in the mobile phase ensures control of the rate of movement of corticosteroids in the column. Since corticosteroids are freely soluble in alcohol, using 90% of this solvent in the mobile phase ensured optimum retention time of these compounds